What is a Centrifuge?

The centrifuge is an instrument that separates fluid of different densities or liquid from solids by applying centrifugal force. To separate the different densities fluid, Centrifuge Puts an object in rotation around a fixed axis (rotate it in a circle), applying a centrifugal force (force perpendicular to the axis of spin). The centrifuge works on the sedimentation principle.

Due to centripetal force denser substances and particles move outward in the radial direction and low-density substances are displaced and move to the center. In laboratory centrifuge samples are kept into the sample tube, the radial acceleration causes the denser particles to settle to the bottom and less dense substances rise to the top.

List of Free Tips and Tricks to Buy the Right Centrifuge

#1)Temperature Control

According to requirement choose between Refrigerated and Non-Refrigerated Centrifuge because sometimes it is required to maintain the sample at a given temperature.

• Refrigerated Centrifuge
• Non-Refrigerated Centrifuge

#2)Process Control

As per necessity how much precision and control required to perform the given process. You can choose between

• Analog Centrifuge
• Digital Centrifuge

#3)RPM or G Force

According to requirement choose the Maximum speed of the centrifuge. Different samples and processes require different speeds to separate particles. Ex: Separation of plasma will be done at 2200-2500 RPM @10 minutes.

#4)Types of Rotor

Mainly two types of rotor heads are used in laboratory centrifuge Fixed Angle and Swing out Rotor. Within these two categories of rotors, various models offer different combinations of capacity and maximum RCF attainable.

#5)Fixed Angle Rotor

It holds the tube at a stable angle (typically 45°) relative to the axis of rotation. When quick pelleting of small particles is required, fixed-angle rotors should be used. Because of their design, these rotors are capable of higher speeds than the horizontal type.

#6)Swing out Rotor (Horizontal Rotor)

When the centripetal force applied to the swing-out rotor it holds the tube at a 90°angle relative to the axis of rotation. Sedimentation of larger particles, such as cells, protein precipitates, antigen-adsorbent complexes, urinary crystal, etc., can be done at lower speeds with horizontal rotors.

#7)Rotor Head Capacity

After selecting the type of rotor head you need to select rotor capacity according to your requirement. It depends on No. of Tubes and the size you require at a time for your requirements. Example of some rotor head capacity are 16 X 15ml, 12 X 15ml, 8 X 15ml, 6 X 50ml etc. In these capacity first digits tell about the no. of tube one rotor head can hold and the second digit tells about the capacity of rotor head like in 16 X 15ml- No of the tube is 16 and each tube capacity is 15 ml.

Various Application of Centrifuge

1. Blood Sample Separation- Blood separates into three main components: red blood cells, white blood cells, and plasma.
2. DNA/ RNA Separation- DNA preparation is another common application for pharmacogenetics and clinical diagnosis.
3. Cell Culture Separation
4. It is used in chemistry, biology, biochemistry and clinical medicine for isolating and separating suspensions and immiscible liquids.
5. Centrifuges are used to separate cream (remove fat) from milk.

Free Safety Tips While Using Centrifuge

The load in a centrifuge must be carefully balanced. When the rotor is at high speed even a small difference in load mass can cause a large force imbalance. Force imbalance may cause damage to rotor, centrifuge or even a person. Some centrifuge comes with an automatic rotor imbalance detection features that immediately stops the centrifuge when an imbalance is detected.

Always check the rotor and lid locking mechanism before starting the centrifuge. Never touch the centrifuge rotor while it’s moving, because it may cause serious injury. Another potential hazard is the aerosolization of hazardous samples during centrifugation. To counteract pollution of the lab, rotor tops with extraordinary airborne tight gaskets are accessible.

The rotor can be stacked with the specimens inside a hood and the rotor top settled on the rotor. A short time later, the airborne tight arrangement of rotor and top is exchanged to the axis. The rotor would then be able to be settled inside the axis without opening the cover. After the run, the whole rotor gets together, including the top, is expelled from the rotator to the hood for additional steps, keeping up the examples inside a shut framework.

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