Sankalp Molisch Reagent for Laboratory Usage, HSMOLSHRG100 (Bottle of 100 ml)

Sankalp Molisch Reagent for Laboratory Usage, HSMOLSHRG100 (Bottle of 100 ml) is a chemical reagent used in organic chemistry and biochemistry to detect the presence of carbohydrates, particularly sugars. It's a crucial tool for identifying the presence of complex carbohydrates, including starches, glycogen, and cellulose.

Key characteristics and uses of Molisch reagent:

Composition: Molisch reagent is a solution of alpha-naphthol in ethanol, typically prepared by dissolving alpha-naphthol crystals in ethanol. It is often referred to as "alpha-naphthol in ethanol" to emphasize its composition.

Carbohydrate Detection: The primary purpose of Molisch reagent is to test for the presence of carbohydrates in a given solution. It reacts specifically with carbohydrates containing free hydroxyl groups (OH), which are a common structural feature of sugars.

Reaction Mechanism: When Molisch reagent is added to a solution containing carbohydrates and heated, it forms a purple to black-colored complex by reacting with the carbohydrate's hydroxyl groups. The reaction involves the dehydration of the carbohydrate and the formation of a furfural or hydroxymethylfurfural compound, which then reacts with alpha-naphthol in the reagent to produce the colored complex.

Interference: Molisch reagent is sensitive to the presence of other compounds with free hydroxyl groups, including phenols and some amino acids, which can lead to false-positive results. Care must be taken to interpret the results correctly in the context of the entire test.

Qualitative Test: The Molisch test is primarily qualitative, indicating the presence of carbohydrates rather than quantifying their concentration. It is often used as a preliminary step in carbohydrate identification.

The Molisch test is conducted as follows:

A small amount of the test solution, typically a liquid or aqueous extract of the substance under investigation, is mixed with a few drops of Molisch reagent.

Concentrated sulfuric acid is carefully added down the sides of the test tube, forming a lower layer. The acid reacts with the carbohydrates, breaking them down into furfural compounds.

The formation of a purple to black ring or interface between the two layers indicates the presence of carbohydrates.

The Molisch reagent test is often used as a complementary tool in the identification of carbohydrates. It is not a standalone quantitative method but a useful qualitative test to indicate the potential presence of carbohydrates in various organic substances, including foods, plant extracts, and biological samples.

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