WKM 3.5 ml Volume and 10 mm Path Length Optical Glass Cuvette KTN193 (Pack of 2 Pcs)

WKM 3.5 ml Volume and 10 mm Path Length Optical Glass Cuvette KTN193 (Pack of 2 Pcs) is a small, transparent, and often disposable vessel used in laboratories for holding and analyzing liquid samples. Cuvettes are typically made of materials like glass, quartz, or high-quality plastics that allow light to pass through with minimal distortion. They are designed to fit into spectrophotometers, fluorometers, and other optical instruments, making them essential tools for various applications in chemistry, biochemistry, molecular biology, and other scientific disciplines.

The primary purpose of a cuvette is to facilitate the accurate measurement of absorbance, fluorescence, or transmission of light through the sample. When a liquid sample is placed inside the cuvette, it becomes the subject of analysis in optical instruments. For example, in a spectrophotometer, the cuvette holds the sample through which light is passed to determine the sample's absorbance or optical density at specific wavelengths.

Cuvettes come in various shapes and sizes, such as rectangular, square, and cylindrical, to accommodate different sample volumes and experimental needs. The choice of material and shape is crucial as they can influence the quality of the results. For instance, quartz cuvettes are used for experiments that require UV light, as they offer superior optical transparency in the ultraviolet range.

One significant advantage of cuvettes is their convenience and ease of use. They are pre-calibrated and readily available in disposable versions, reducing the risk of cross-contamination between samples and eliminating the need for extensive cleaning procedures.

In laboratories, cuvettes find applications in a wide range of experiments:

Spectrophotometry: Cuvettes are used for measuring the absorption spectrum of a sample, allowing researchers to quantify the concentration of absorbing compounds.

Fluorescence Spectroscopy: Cuvettes hold fluorescent samples in fluorometers to study their emission spectra and measure fluorescence intensity.

Biochemical Assays: Cuvettes are used to perform various biochemical tests, such as enzyme assays, protein quantification, and DNA concentration determination.

Cell Culture and Microbiology: In cell culture and microbial studies, cuvettes hold liquid samples for optical density measurements, cell counting, and microbial growth assessments.

To ensure accurate and reproducible results, it is essential to handle cuvettes properly and keep them clean and free from scratches or other defects that could affect the light transmission.

In conclusion, cuvettes are indispensable tools in laboratories for optical analysis and measurements of liquid samples. Their optical transparency, various shapes, and convenience make them versatile instruments used in spectrophotometry, fluorescence spectroscopy, and other applications. With cuvettes' precision and reliability, researchers can gain valuable insights into the properties and characteristics of the substances under investigation, advancing scientific knowledge in diverse fields of study.

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