Tarson 35 µl PP 384 Well A24 - ABI Type Cut Crner PCR Plate 613070 (Pack of 50 Pcs)

Tarson 35 µl PP 384 Well A24 - ABI Type Cut Crner PCR Plate 613070 (Pack of 50 Pcs) is a widely used technique in molecular biology laboratories for amplifying specific DNA sequences. It is a crucial step in various applications such as gene expression analysis, DNA sequencing, and genetic testing. To perform PCR, a PCR plate is used as a reaction vessel to hold the PCR reaction mixture.

A PCR plate is a plastic plate with multiple wells or tubes arranged in a grid pattern. The most common format is a 96-well plate, but plates with 384 or 1,536 wells are also available for high-throughput applications. Each well in the plate can hold a small volume of the PCR reaction mixture, typically ranging from 10 to 100 microliters.

The PCR plate is designed to withstand the thermal cycling process involved in PCR. It is made of a special type of plastic, usually polypropylene, which is resistant to high temperatures and does not interfere with the PCR reaction. The plate is also optically clear, allowing for easy visualization of the reaction mixture during loading and analysis.

To use a PCR plate, the first step is to prepare the PCR reaction mixture. This typically includes the DNA template, primers, nucleotides, buffer, and DNA polymerase. The reaction mixture is then dispensed into the wells of the PCR plate using a multichannel pipette or automated liquid handling system.

After loading the reaction mixture, the PCR plate is sealed with a compatible sealing film or cap to prevent evaporation and contamination. The plate is then placed in a thermal cycler, which heats and cools the reaction mixture to facilitate the amplification of the DNA target sequence. The cycling parameters, including the temperature and duration of each step, are programmed into the thermal cycler based on the specific PCR protocol.

Once the PCR amplification is complete, the PCR plate can be used for various downstream applications. This may include DNA sequencing, gel electrophoresis, or further analysis using other molecular biology techniques.

In summary, a PCR plate is an essential tool in molecular biology laboratories for performing PCR. It provides a convenient and efficient platform for conducting PCR reactions in a high-throughput manner. The plate is designed to withstand the thermal cycling process and is compatible with various downstream applications, making it an indispensable component in PCR workflows.

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