Sankalp Neubauer Chamber for Laboratory Usage, HSNEUBARCHMBR

Sankalp Neubauer Chamber for Laboratory Usage, HSNEUBARCHMBR is a specialized glass counting chamber used in laboratories for accurate cell counting, particularly in biological and biomedical fields. It is an essential tool for determining the concentration of cells or microorganisms in a liquid sample, allowing scientists to calculate cell density, viability, and other important parameters.

Key features and functions of the Neubauer chamber:

Construction: The Neubauer chamber is a square-shaped glass slide with a shallow rectangular depression in the center. This depression is marked with a grid pattern, which is etched or engraved on the glass surface.

Grid Pattern: The grid pattern on the Neubauer chamber consists of lines and squares that divide the central area into smaller squares or counting areas. These grids are essential for accurate cell counting.

Counting Area: The counting area is a precisely measured section of the chamber where cells or particles can be counted. The size and volume of this area are known, allowing for accurate cell concentration calculations.

Volume Chambers: A Neubauer chamber typically comes in two versions, one for counting smaller volumes of liquid and another for larger volumes. The smaller chamber is called the "depth of 0.1 mm," while the larger chamber is called the "depth of 0.2 mm." These differences in depth affect the volume of liquid sampled and the area to be counted.

Using a Neubauer chamber involves several steps:

Sample Preparation: A small sample of the liquid containing the cells or microorganisms is loaded into the chamber, typically using a pipette or micropipette.

Microscope Observation: The Neubauer chamber is placed under a microscope with the grid pattern facing up. Under the microscope, the cells in the counting area become visible.

Cell Counting: The cells or particles in the grid squares are manually counted, and the total count is recorded. Typically, only the cells touching the top and left boundaries of each square are counted to avoid duplication.

Calculation: By knowing the volume of liquid in the counting chamber and the number of cells counted, the cell concentration is calculated.

Neubauer chambers are invaluable tools for a wide range of applications, including cell culture, microbiology, and medical diagnostics. They provide a cost-effective and straightforward method for determining cell density and viability, allowing researchers and clinicians to monitor and control cell growth and population dynamics in various experiments and clinical tests.

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